Fluoview fv mpe multiphoton microscope product manuals. Region of interest roi scanning and bleaching using. Retain this manual in an easily accessible place near a system for future reference. Fluoview fv mpe multiphoton microscope software downloads. A, part of the general information in the metadatafile, right below confocal on as an input in the form below. Olympus fluoview fv is designed for highresolution, confocal observation of both fixed and living cells. This free pc software was developed to work on windows xp, windows vista, windows 7, windows 8. This software will read the data captured by a fv fv500fv300. To ensure the safety operation of laser system, we recommend you to study the manual of each laser and the light source equipment besides this manual. Olympus fluoview fv bx2 upright confocal university.
Olympus fluoview this is part of the backprojected pinhole calculator. With usersavable and selectable software work ows, the system adjusts to individual needs. The olympus fluoview fv is a nextgeneration imaging system designed for highresolution, confocal observation of both fixed and living cells. If this program is not available in your pc or mac, you can download it for free from adobe. The microscopy and imaging center is a core user facility supported by the office of the vice president for research. Fluoview fv mpe multiphoton microscope back to fluoview fv mpe multiphoton microscope product. Refer to the lens cleaning poster if you need any help recalling the rules and steps.
Olympus fv3000 rs fluoview software demo module 1 youtube. Prior to using this microscope, read this instruction manual for sure to utilize fullfledged performance of this microscope and ensure customers safety. Olympus fluoview fv download your lost manuals for free. Images saved with the fv file format oif, oib, fv multitiff can be read. Utilizing pulsed ir lasers, the olympus fluoview mpe is able to image hundreds of microns into a specimen thanks to the penetration of. Included in this section are brochures on research microscopes, digital camera systems, laser systems, objectives, dichromatic mirror units, software, and research microscopes. Olympus fv multiphoton manual duke light microscopy. Utilizing pulsed ir lasers, the olympus fluoview mpe is able to image hundreds of microns into a specimen thanks to the penetration of ir light and our long working distance objectives. Retain this manual in an easily accessible place near the work desk for future reference. Operation instructions describes the operation procedures of the fluoview system, for example, methods for image acquisition and various image processing. Back to fluoview fv mpe multiphoton microscope product. Full text of olympus fluoview fv microscope and magnifier user manual see other formats olympus your vision, our future confocal laser scanning biological microscope fv1 000 fluoview worldleading optic flu oview always evolving flu 0 view from olympus is open fluoview more advanced than ever the olympus fluoview fv confocal laser scanning microscope delivers.
Postbox 10 49 08, 20034 hamburg, germany wendenstrasse 1418, 20097 hamburg, germany phone. Fluoview fv confocal microscope from olympus biocompare. May 03, 2018 a detailed overview and tutorial for systematically navigating through olympus fluoview software for operating olympus fv3000rs microscope. The mic is staffed by research scientists with expertise in transmission electron microscopy, scanning electron microscopy, light microscopy, and supporting instruments therein. By incorporating high dynamic range imaging hdri, a greater dynamic range of fluorescence between the lightest and darkest areas of a sample is obtained. Select desired colors from the dye box by clicking on the name of the dye and dragging the ball to the open square above.
Functions are executed by selecting the panel page tab at the edge of the panel of the function to be executed and the panel will move to the front. The fv offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging, with minimal risk of damage to living specimens. Fluoview enables precise, bright imaging with minimum phototox two versions available. Simplified operation manual a x 7 9 2 3 type 2 this manual introduces a simpli. Petition thank you for your purchase of olympus microscope at this time. Interactive java tutorials laser scanning confocal microscope simulator. The illumination area is not limited to the imaging field of view, but covers areas outside as well. The software can control the motorized stage to tile together stacks of images to allow imaging of a large area. Olympus fluoview fv microscope and magnifier user manual. This enables selective illumination in user configurable rois which can for example be used for photobleaching, cutting, ablation, micro manipulation and uncaging. Olympus fluoview fv500 laser scanning confocal system. Operation instructions describes the operation procedures of the fluoview system, for example, methods for. Olympus fv3000 rs fluoview software demo module 5 youtube.
The acquisition parameters are the same for each xy position so the first thing to do is set the zstack and all settings as you would normally but suitable for all the xy positions. Olympus software manual centre for microscopy and imaging at. Olympus fluoview 300 user guide university of north. The olympus fluoview fv confocal laser scanning microscope delivers efficient and reliable performance together with the high resolution required for multidimensional observation of cell and tissue morphology, and precise molecular localization.
The facility manager tracking software makes it easy to track system. It is based on a fully motorized olympus ix81 inverted microscope. Olympus dp73 instruction manual instruction manual 60 pages olympus fluoview fv overview overview 28 pages olympus microscope. This us ers m anual is for the software to be run on o ly mpus fluoview fv300 confocal las er. Use the username and password provided to you to log on to the computer. Page 3 caution caution fvmpe is a class 4 laser product. The fvs sim system can provide excitation scanning separately from image scanning. Safety and hardware as well as the navigationonline help of the software.
Fluoview manual configuration the fluoview system uses two manuals including this users manual and the onscreen manual built into the software online help. Olympus fluoview fv mpe, a multiphoton laser scanning microscope that allows fluorescence imaging deep within specimens. Tutorials has a lot of tips on deciding imaging parameters. Olympus fluoview fvmpe, a multiphoton laser scanning microscope that allows fluorescence imaging deep within specimens. The fv an ideal choice for frap, flip and photoactivation. Data saved as tiff and avi can be easily used and exported. Safety guide describes notes and cautions on using the fluoview system and on types of.
The fluoview fv3000 series is designed to meet some of the most dif. For detailed information about the olympus fluoview tm microscopes and available support equipment for the innovative microscopist, the appropriate brochures may be downloaded in this section. Olympus fluoview fv mpe multiphoton microscope user manual. Single fibertype combiner is used for main scanner fv with up to six lasers, ranging from 405 to 635 nm. The olympus fluoview tm fv is a nextgeneration imaging system designed for highresolution, confocal observation of both fixed and living cells. Fluoview fv and the instruction manual of the microscope in order to understand overall operation methods. When decompression completes, a dialog box to confirm your action will be displayed. The olympus fluoview fv has a userfriendly image acquisition and image analysis software as well. Users manual hardware manual fluoview fv confocal laser scanning biological microscope fv10asw ver. Users manual quick start fluoview fv laser scanning biological microscope fv10asw ver2. Fluoview fv3000 service available, accessible, continuous support this brochure illustrates the portfolio of principle services available from olympus. The fv offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens.
For detailed operating procedures, refer to the separate user. This users manual is for the olympus fluoview fv confocal laser. Note if you are fv10sw user, dont double click the fluoview setup icon on the desktop and boot it up while the fluoview software is running. This users manual is for the software to be run on olympus fluoview fv confocal laser. Ax7797 hold this manual by your side when using this microscope all the time and keep it with care after reading. This free pc software was developed to work on windows xp, windows vista, windows 7, windows 8 or windows 10 and is compatible with 32bit systems. Fluoview tm fv confocal laser scanning biological microscope a leader in the next generation of live cell confocal imaging systems, the fv features. Retain this manual in an easily accessible place near a system for future. Olympus fluoview fv mpe multiphoton microscope user. Quick start fluoview fv confocal laser scanning biological microscope fv10asw. This users manual is for the software to be run on olympus fluoview fv500 confocal laser. This software will read the data captured by a fvfv500fv300. The fluoview software is organized by two kinds of panels, the function panels left, down side and display panel big one on right using the fluoview.
All microscope control must be through the fluoview software windows, figures 2. Since this software is for reading, image capture, measurement, analysis, 3d function etc. Fluoview fv3000 laser scanning microscope the fluoview fv3000 series is designed to meet some of the most dif. I found that one can easily master the software with limited training time. View and download olympus fluoview fv user manual online. Sep 30, 2009 the olympus fluoview fv has a userfriendly image acquisition and image analysis software as well. New updates to the olympus fv10asw software extend. Preparation for operation starting the software 12 starting the software turn on the microscope and power supply units before starting this note software. Perhaps the most significant advance in optical microscopy during the past decade has been the refinement of mainstream laser scanning confocal microscope lscm techniques using improved synthetic fluorescent probes and genetically engineered proteins, a wider spectrum of laser light sources coupled to highly accurate. With the high sensitivity and speed required for live cell and tissue imaging and the ease of use and exibility required for. Hamburg, germany, june 8, 2011 olympus europa holding gmbh has introduced fv10asw 3. Click the unzip button to start to decompress the downloaded file. Olympus fv confocal microscope microscopy and imaging. The users manual is composed of the two following volumes and subject matter.
Technical personnels who perform the service require to take the service training. The users manual is composed of the five following volumes and subject matter. Fluoview tm and support equipment brochure downloads. Fv laser scanning biological microscope fv10asw ver2. References 1 coupling of pulsed laser sources into the olympus fluoview fv300 fv using a polarization beam. Full text of olympus fluoview fv microscope and magnifier user manual see other formats olympus your vision, our future confocal laser scanning biological microscope fv1 000 fluoview worldleading optic flu oview always evolving flu 0 view from olympus is open fluoview more advanced than ever the olympus fluoview fv confocal laser scanning microscope delivers efficient and. Part or whole of this software as well as manual shall not be used or duplicated without. Fluoview fv mpe multiphoton microscope change operating system. Olympus fluoview fv500 training university of minnesota. A, part of the general information in the metadatafile, right below confocal on as. On the following screen, enter your unique user name and password, and click ok.
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